About project
Loggerhead sea turtle (Caretta caretta) microbiome: insight into endozoic and epizoic communities
(Mikrobiom glavate želve (Caretta caretta): uvid u epizoičke i endozoičke zajednice)
TurtleBIOME project contribution is to provide a clear, complete and detailed image of the microbial composition of the surface biofilm and the gut microbiome of loggerhead sea turtles. from the Mediterranean Sea. We plan to achieve this goal by pairing state-of-the-art culture-independent molecular approach, e.g. new generation sequencing (NGS) of prokaryotic and eukaryotic components of the micro-community with classical approach of microorganism cultivation, experiments and light and electronic microscope observations of biofilm components.
Morphological analyses of turtle-associated surface biofilms
Light and electron microscopy
Cultivation of microorganisms
Establishment and characterization of monocultures and co-cultivation experiments
TurtleBIOME Diatom strain information
During the project, we have successfully cultured 181 diatom strains. These strains have been taxonomically curated and assembled in a library of reference DNA sequence data and morphological information. The data published here were assembled by Toms Ekmanis and made available to the public on this webpage. All requests and questions please send to suncica.bosak@biol.pmf.hr
SEM and LM images are also available upon request. The GenBank accession numbers for SSU/rbcL/psbC sequences are listed in the Table. Some of the strains are deposited also in the BCCM/DCG Diatoms Collection and ID numbers are also listed.
The complete information is available here:
TurtleBiome_Diatom_Strains_Information_Table
TurtleBiome Full Phylogenetic tree
Culture-independent molecular characterization
Prokaryotic & eukaryotic microorganisms: amplicon based and metagenomic approach
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ABOUT US
TurtleBIOME installation research project aims to combine efforts of young and competent scientists in a single cohesive group that will set out the foundation for an interdisciplinary research approach in fields of marine ecology, marine biology and conservation. Within our research group we combined expert knowledge on turtle biology, diatom taxonomy, microbial ecology and bioinformatics. Furthermore, we aim to develop Croatian research skills and expertise through focus on training of young scientists in cutting edge methodology combined with national and international network building.
PhD student / Postdoctoral researcher
Marine Microbial ecology group
During the pandemic and lockdown period, our group meetings were happening in a safe virtual environment.
So here we are, TurtleBIOME group in an online meeting in our third year of the project!
